| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1208-1 | ||||
Resumo:Microorganisms are essential for the environment, as they contribute to the stability of ecosystems, participating in various biogeochemical cycles. As far as the aquatic ecosystem is concerned, these organisms participate in the decomposition of organic matter into nutrients and in the control of water quality. However, this environment has been significantly altered due to the urbanization process, modifying the structure and diversity of the local microbiota. The Cavouco stream corresponds to an impacted urban stream which receives a polluting load of waste from teaching and research laboratories, health service waste, in addition to domestic waste from the surrounding population. This process generates a cumulative effect of these residues, which has been causing changes in the dynamics of this ecosystem, altering the quality of the water and contributing to the occurrence and dissemination of pathogenic bacteria, since it flows into one of the main rivers in the state of Pernambuco, the Capibaribe River, which is used for local agriculture and fishing. The present study aimed to characterize the microbiota of an impacted aquatic environment in Recife-Pernambuco, Brazil. Water and sediment samples were collected at three different points, and total DNA extraction was performed using the “DNeasy PowerSoil Pro Kit” extraction kit. They were quantified and then used in the construction of 16S rRNA libraries (V3-V4 regions). Then, they were sequenced on the Illumina MiSeq platform using the “Paired-end” protocol (2x300bp). Analysis of the taxonomic structure showed that the environment is dominated by bacteria (99.3%) compared to archaea (0.7%). A total of 22 bacterial phyla were observed in this environment, in which there was a predominance of Proteobacteria (44.14%), Bacteroidetes (11.83%) and Firmicutes (11.65%). The most abundant genera were Methylobacter (2.40%), Geobacter (2.29%), Coprothermobacter (2.20%), Povalibacter (2.20%), Clostridium (1.96%), Anaeromyxobacter (1 .93%) and Thiobacillus (1.51%), with a high number of unclassified genera (51.38%) worth noting. Considering the approach, it can be concluded that the sampling of Riacho Cavouco has a wide diversity of pathogenic microorganisms that are related to environmental impacts and risks to human health, highlighting the importance of constant and effective monitoring of the local microbiota. Palavras-chave: bacteria, monitoring, pollutants Agência de fomento:Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). | |||||